Date: 11-12 December 2017
Venue: Royal Museum for Central Africa, Leuvensesteenweg 13, 3080 Tervuren, Belgium.
Programme:
Monday 11th. 14:00 - 17:00
Plenary lecture "Mitochondrial metagenomics"
Tuesday 12th. 09:30 - 17: 00
Hands-on sessions:
Session 1a: De novo assembly of a whole mitochondrial genome from illumina paired sequencing reads
- Introduction to assembly and the linux command line
- Quality control, adapter trimming and read pairing
- Mitogenome assembly using two different types of assemblers
Session 1b: Assembly interrogation and annotation
- Viewing and assessing assembly quality
- The effects of variation of assembly parameters
- Annotating assembly using local and web tools
Session 2a: Assembling with sparse and low quality data
- Selection of reference sequences
- Assembly to a reference sequence
- Viewing and annotating incomplete mitogenomes
Session 2b: Mitochondrial metagenomics
- Assembly of mixed sequence libraries using multiple assemblers
- Meta-assembly of multiple results
- Contig identification
For the hands-on sessions, we will accept a maximum number of 30 participants. We still have a few slots available and we will select at least one person per research unit.
For the practical sessions, you'll only need to have a few applications installed on your laptop :
- SSH client
Windows: PuTTY www.putty.org
Mac/Linux: not required, terminal should be installed as standard
- FTP client - transfers files to/from the server
Windows/Mac/Linux - Filezilla Client www.filezilla-client.org
- Assembly viewer - visualisation of assemblies and coverage
Windows/Mac/Linux: Tablet ics.hutton.ac.uk/tablet/
- Sequence viewer - visualisation and manipulation of sequences and annotations
Windows/Mac/Linux: UGENE ugene.net
